So every year I would do the "Toothpickase" lab. I hated wasting ALL the toothpicks and also of course students would end up trying to stab each other and put toothpicks in each other's hair, but I still thought it was a good use of time. However, since we can't use shared materials this year and am NOT buying enough for every student, I converted it to a virtual lab. Personally, I think this is a better lab than the actual Toothpickase lab since I made this more realistic using a real enzyme, maltase. I don't think I will go back to doing the Toothpickase lab after this! If you have never done that lab, it really helps students comprehend reaction rate and how different changes in the reaction can affect the reaction rate. In order to understand this lab, student should have completed my previous lesson Enzyme Basics. So here is the breakdown of the lab: Review: Students review the parts of the enzyme reaction and the vocabulary needed to understand this lab (enzyme, substrate, active site, product). Background: Students are given background on the maltase enzyme and how it works. Part A: Students are instructed to count the number of glucose molecules they see in the reaction. They then record it in the data table. Students do this at different time intervals during the reaction. Students then graph their results and answer questions. Part B: Students are instructed to count the number of glucose molecules they see in the reaction. They then record it in the data table. Students do this at different time intervals during the reaction. Students then graph their results and answer questions. The difference is during this section, the substrate concentration has decreased. Part C: Students are instructed to count the number of glucose molecules they see in the reaction. They then record it in the data table. Students do this at different time intervals during the reaction. Students then graph their results and answer questions. The difference is during this section, the enzyme concentration has doubled. Compare graphs: Students then paste all 3 graphs they have completed on this slide and compare and contrast the graphs. They draw some biological conclusions based on the data. Topics addressed in this lab include how decreased substrate concentration and increased enzyme substrate concentration affect reaction rate. Other topics addressed include inhibitors, the fact that enzymes themselves do not "go faster", that when enzymes run out of substrate, the reaction will stop, the best way to speed up chemical reactions and how change in temperature affects the enzyme. Interested in this lesson? Check out my TpT store! Also check out my "search" feature to see other available lessons. New lessons are added weekly!
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August 2021
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